Fascination About attune flow cytometer
Fascination About attune flow cytometer
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Details-enriched characterization of cell populations. Morphological details from photos can increase on the richness of flow cytometry knowledge. One example is, the determine exhibits an otherwise regular apoptosis assay employing Annexin V and PI, including cell imaging to characterize cells in Just about every populace to reveal morphologically distinctive functions. These insights could not happen to be gained from multiplex staining alone.
As samples are obtained around the Attune CytPix Flow Cytometer, the large-velocity brightfield digital camera captures and shops visuals of detected events at a fee nearly 6,000 images per next, based on the flow price and image dimensions. For increased versatility, Attune Cytometric Software package permits you to regulate the picture capture frequency as essential and choose a certain gate for picture seize, if needed.
(B) RAW 264.7 macrophages tend to be annotated inaccurately with regard to particle depend due to their complexity and noncircular borders. The volume of singlets obtainable for Assessment raises after the user trains and employs a far more exact design.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing which has a superior-speed brightfield digital camera for simultaneous higher throughput flow cytometry and significant resolution brightfield imaging.
"A picture is not very truly worth a thousand dots, however it genuinely helps you to see images. It is excellent to discover illustrations or photos that correlate with staining. For example, AnnexinV-favourable cells that are morphologically distinctive than balanced cells, or CD14+ monocytes which can be larger and much more textured than CD3+/CD4+/CD14- lymphocytes.
In this article, a highly trained person has gated singlets confidently. Immediately after analyzing the manual singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate incorporates a lot more than 4% aggregates.
Optimizing gating strategy. Even sturdy manual singlet gating is error-inclined and stays a subjective choice place in Nearly all flow cytometry assays. Imaging may be used to substantiate and alter gates to include only single cells of interest.
Our scientists will design and style panels in your case as often times you would like—gratis, no solution acquire required.
The Attune NxT cytometer may be the dependable workhorse instrument your lab can depend on for dependable and dependable overall performance. The Attune CytPix product incorporates a high-speed brightfield digicam and AI-driven impression Assessment software program, with new updates including equipment Mastering applications and an intuitive user interface, enabling Improved data precision, accuracy, and faster insights into sample biology.
Maybe most of all, these activities contain cells of Obviously diverse phenotypes which could lead to incorrect conclusions concerning double good gatherings (particularly in rare populations).
Seem waves help more info precise mobile alignment, leading to Extraordinary details excellent and ~10X a lot quicker sample flow fees.
Application Take note: Automated picture Assessment reduces user-to-consumer variability in flow cytometry gating strategies
Leverage the power of advanced technological innovation and innovation to accelerate your discoveries. Have confidence in in the Attune Flow Cytometer spouse and children to deliver the trustworthiness, pace, and precision that set your work apart.
Versatile payment plan from Agilent Fiscal Answers assist you to get the crucial instrumentation you may need for immediate task, or to develop your lab.
Gating on these extended parameters permits you to immediately and precisely detect populations of interest and confirm gating technique with little or no guide evaluate. The Attune program presents usage of condition-of-the-artwork machine Finding out Evaluation tools within just an expertly made user interface, which makes it exceptionally user friendly.
The Attune Xenith cytometer is often a substantial-parameter spectral analyzer, delivering equally spectral unmixing and conventional flow capabilities for comprehensive and multipurpose cell analysis.
Agilent Worth Promise guarantees you at least 10 years of instrument use through the date you purchase. If not, We're going to credit score you While using the residual worth of the program towards an upgraded design.
Below, we present that by gating procedures using image-dependent quantitative parameters can distinguish interacting cells from coincident events to more correctly assess interacting cells.